Journal: Bioactive Materials

Article Title: A catalytically active and recyclable bioelastomer inspired by metalloenzymes

doi: 10.1016/j.bioactmat.2026.02.053

Figure Lengend Snippet: Host responses to mouse subcutaneous implantation of Cu-PIAS. ( A ) Representative hematoxylin and eosin staining of the implant site after 7, 14, and 21 days. 25-Cu-PIAS mesh is roughly centered in each image and runs horizontally. Mesh material stains homogenously red and is indicated by arrows in the leftmost image of each timepoint. ( B ) Area of 25-Cu-PIAS mesh at each timepoint. Reduction over time indicates degradation of material. ( C ) Blood vessel density around and within the implant sites (CD31 immunohistochemistry) at each timepoint. ( D ) Total macrophage density around and within the implant sites (F4/80 immunohistochemistry) at each timepoint. ( E ) M2 macrophage density around and within the implant sites (CD163 immunohistochemistry) at each timepoint. ( F ) Ratio of macrophages expressing a M2 phenotype at each timepoint. ( G ) Collagen thickness above the implant site of scarring mouse model (CXCR3 −/− ), comparing responses to 15-Cu-PIAS and fibrin gel after 3 and 6 months. Statistics: ∗, ∗∗, ∗∗∗, and ∗∗∗∗ indicate p ≤ 0.05, 0.01, 0.001, and 0.0001, respectively.

Article Snippet: F4/80 antibody was purchased from Cell Signaling Technology (Danvers MA, Cat #: 70076).

Techniques: Staining, Immunohistochemistry, Expressing